| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Beeson, Hadley C (14)
|
NTESP |
4:39.69 |
4:43.45 |
3.76 |
| 2 |
Hosch, Lindsey N (14)
|
NTESP |
4:46.43 |
4:53.50 |
7.07 |
| 3 |
Fuhs, Emma K (14)
|
VATIDE |
5:25.00 |
5:00.53 |
-24.47 |
| 4 |
Beeson, Abigail D (13)
|
NTESP |
5:09.13 |
5:06.68 |
-2.45 |
| 5 |
Gill, Virginia A (14)
|
VATIDE |
5:42.71 |
5:16.95 |
-25.76 |
| 6 |
Moseley, Aileen C (13)
|
NTESP |
|
5:28.76 |
- |
| 7 |
Swaim, Ashlyn T (13)
|
VATIDE |
|
5:35.90 |
- |
| 8 |
Tadder, Shannon A (13)
|
VATIDE |
5:47.72 |
5:39.97 |
-7.75 |
| 9 |
Donahue, Sarah C (14)
|
VATIDE |
6:08.03 |
5:44.59 |
-23.44 |
| 10 |
Stidham, Catherine E (14)
|
NTESP |
|
5:48.44 |
- |
| 11 |
Pogorzelski, Megan G (13)
|
VATIDE |
|
5:49.19 |
- |
| 12 |
Knoop, Autumn E (13)
|
VATIDE |
5:54.79 |
5:50.30 |
-4.49 |
| 13 |
Ziemer, Ruby A (13)
|
VATIDE |
|
5:51.74 |
- |
| 14 |
Hudson, Ava K (14)
|
MDHFY |
6:00.47 |
5:52.78 |
-7.69 |
| 15 |
Carlile, Karisma L (14)
|
VATIDE |
|
6:25.87 |
- |
|
Wilson, Brianna L (14)
|
VAYOBX |
5:19.00 |
NS |
- |